Portable Dna Detection System Based on Ultrafast Segment-flow and Fluorescence Detection

To detect the biological agents, the DNA detection is most usable due to the sensitivity and the selectivity. Although the key technology of DNA detection is real-time polymerase chain reaction (PCR), which uses exponential amplification of PCR and real-time detection of the amplified DNA fragments, the long thermal cycling time has been the problem to apply the rapid detection of pathogenic organisms. We developed a portable and rapid detection system based on ultrafast segment-flow PCR and fluorescent detection. Using the portable system, a model microorganism of biological warfare agent was successfully detected within 6.5 min with an enough high sensitivity.